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The rugose variant of V. cholerae classical biotype (sixth pandemic) strain NCTC 6585 produces an extracellular glycocalyx. TEM of ruthenium red-stained thin sections of smooth and rugose cells of classical strain NCTC 6585. Micrographs show no stained matrix between smooth cells (left panel) and densely stained matrix between rugose cells (arrow, right panel).
