FIG. 3.

Biofilm formation by smooth and rugose colony variants of V. cholerae. Glass test tubes containing 500 μl of LB broth were inoculated with a 1:100 dilution of overnight cultures of N16961, NCTC 6585, and Aldova O37 strains and incubated statically at room temperature for 24 h. Quantitative biofilm assays were performed as described previously (24).