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. 2026 Feb 27;29(4):115137. doi: 10.1016/j.isci.2026.115137

Figure 1.

Figure 1

Differential expression of OCT4A and PG1 in uterine cancer cell lines

(A) OCT4 and PG1 gene expression data from cancer cell lines were registered in the Public 24Q4 data of the Cancer Cell Line Encyclopedia (CCLE). The Figure plots the data from representative lineage cell lines.

(B) Scatterplot of the correlation between OCT4A and PG1 expression. Pearson’s correlation coefficient between OCT4A and PG1 across all cell lines was 0.207. The correlation within the bowel lineage alone was 0.359, and within the uterine lineage alone, it was 0.126. In the combined group of the testes, ovary/fallopian tube, kidney, uterus, esophagus/stomach, and bowel, the correlation was 0.06364, indicating a lack of correlation between the two parameters.

(C) Evaluation of OCT4 and PG1 gene expression levels in normal and cancerous tissue samples from patients with uterine and colorectal cancers using Gene Expression Omnibus 2R (GEO2R) against the GSE146889 RNA-sequencing database.

(D) Sashimi plots of samples with high OCT4 and PG1 expression in GSE146889. Sashimi plots were used to visualize splice junctions derived from the aligned RNA-seq data along a gene annotation track. In these plots, the coverage of each alignment track is represented by a bar graph. Arcs connect exons to indicate splice junctions, and the number of reads spanning each junction is displayed on the arcs.

(E) The relative mRNA levels of OCT4A and PG1 were measured using real-time PCR with specific primers (Figures S1D and S1E). Data are presented as relative expression levels normalized to HPRT1, with HPRT1 set to 1. n = 3. Mean ± SD.

Data from colorectal cancer and ovarian germ cell tumor cell lines, where high OCT4A expression is known, are shown in Figure S2.

(F) Validation of OCT4A-positive endometrial cancer cell lines using tagged genomic transgenes. Immunocytochemistry was performed using an anti-FLAG antibody on cells that were transfected with pOCT4AGen-FLAG. Anti-FLAG antibodies and RFP markers were used to identify FLAG-tagged OCT4A protein-positive and vector-transfected cells, respectively (Figure S3). Proportion of FLAG/RFP-positive cells in each cell line. The percentages of FLAG-positive cells relative to RFP-positive cells were 0.05, 3.83, 3.08, and 13.3% in Ishikawa, HEC265, HEC151, and HEC50B cells, respectively. n = 3. Mean ± SD.