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. 2026 Feb 27;29(4):115137. doi: 10.1016/j.isci.2026.115137

Figure 6.

Figure 6

PG1 proteins can stabilize and exhibit transcriptional activity by dimer formation with OCT4A and SOX2

(A) Evaluation of OCT4A-OCT4A and PG1-PG1 homodimer formation, as well as PG1 heterodimerization ability with OCT4A and Sox2, using the coral hue assay in HEK293A cells. Scale bars, 50 μm.

(B) Evaluation of the transcriptional regulatory activity of the co-expression of OCT4A and PG1 in the dual-luciferase assay when increasing the amount of PG1 in COS7 cells. n = 3. Mean ± SD.

(C) Expression levels of PG1 protein when co-transfected with OCT4A or SOX2 in COS7 cells. Scale bars, 100 μm. The results of protein detection using western blotting are shown in Figure S6.

(D) Expression level of PG1 protein in the presence of OCT4A or SOX2 using the P2A peptide for co-expression of the two genes in COS7 cells. Scale bars, 100 μm. The results of protein detection using western blotting are shown in Figure S6.

(E) Left: Detection of forcibly expressed PG1 protein in various cell lines (PA1, HEC50B, Ishikawa, and MCF7) with different OCT4A expression levels. Scale bars, 100 μm. Right: Expression levels of OCT4A and SOX2 mRNA in the cell lines used in the CCLE database.