Figure 10.
An essential role of GS15 in the Golgi apparatus. Hela cells were transfected with GS15-specific siRNA and then analyzed by immunofluorescence microscopy to detect GS15 and other indicated markers of the Golgi apparatus. (A) cis/medial proteins GS28, syntaxin 5, and Ykt6 were redistributed into small dotty and diffuse labeling in cells whose GS15 levels were significantly reduced by siRNA. (B) Even trans-Golgi/TGN β1,4-galactosyltransferase (GT), TGN syntaxin 6, and Golgi matrix GM130 were noticeably affected by GS15 knockdown. Bars: 10 μM.