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. 2002 Oct;13(10):3493–3507. doi: 10.1091/mbc.E02-01-0004

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Copyright © 2002, The American Society for Cell Biology

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Reciprocal coimmunoprecipitation of GS15, syntaxin 5, GS28 and Ykt6. A: Golgi detergent extracts were immunoprecipitated with antibodies against GS15 (lane 1) or control antibodies (lane 2). The immunoprecipitates of anti-GS15 (lane 1) or control antibodies (lane 2) and 10% of the starting material (lanes 3) were resolved by SDS-page and processed for immunoblot to detect the respective proteins as indicated on the left. As shown, syntaxin 5, GS28 and Ykt6, but not Bet1, Sec22b or syntaxin 6 were coimmunoprecipitated by anti-GS15 antibodies. B: Golgi detergent extracts were immunoprecipitated with antibodies against GS28 (lane 2), syntaxin 5 (lane 3), Ykt6 (lane 4), or control antibodies (lane 1). The immunoprecipitates, along with 10% starting material (S) were resolved by SDS-page and analyzed by immunoblot to detect the respective proteins as indicated on the left. C. Golgi detergent extracts were immunoprecipitated with antibodies against syntaxin 5 (lanes 1 and 3) or control antibodies (lanes 2 and 4). The immunoprecipitates (lanes 1 and 2) and 10% supernatants (lanes 3 and 4) as indicated were resolved by SDS-page and processed for immunoblot to detect the indicated proteins.