Figure 7.

Coimmunoprecipitation of components of COPI coat by anti-GS15 antibodies. A: Golgi detergent extracts were immunoprecipitated with control antibodies (lane 1) or anti-GS15 antibodies (lane 2). The immunoprecipitates were resolved by SDS-page. After staining with Coomassie-blue, the proteins bands immunoprecipitated by anti-GS15 were excised for amino acid sequence identification. The amino acid sequences of the indicated proteins were shown on the right. B. Golgi detergent extracts were immunoprecipitated with antibodies against GS15 (lanes 1), anti-Bet1 (lane 3), or control antibodies (lanes 2 and 4). The immunoprecipitates were resolved by SDS-page and processed for immunoblotting with antibodies against β-COP.