Figure 8.

Expression of GS15 mutant without TM (GS15ΔTM) disrupts normal Golgi distribution of syntaxin 5, GS28, and Man II, while the Golgi distribution of Vit1-rp2/Vit1a, syntaxin 6 and Golgi matrix GM130 and p115 were less affected. NRK cells were transiently transfected with a construct for expressing GS15ΔTM. Transfected cells were fixed and processed for indirect immunofluorescence to detect the expressed mutant GS15 (left panels) and indicated Golgi proteins in central panels. The merged images are presented. Bar, 10 μM.