Figure 2.

Transgene expression and analysis of DNA integrity in lactating and involuting mammary glands. (A) Western blotting performed with protein extracts from 7-d-lactating and 2-d-involuting mammary glands by using an antibody against the β1-integrin cytoplasmic domain. Arrows indicate positions of endogenous β1-integrin and transgenic protein in the gel. β-Actin was used as loading control (bottom). (B) Double indirect immunofluorescence staining of a section from a 2-d-involuting transgenic gland with an anti-CD4 monoclonal antibody (left) and a polyclonal anti-laminin antibody (right). (C) Whole-mount staining of 4-d-involuting wild-type (left) and transgenic (right) glands. Bar, 25 μm for B; 2 mm for C. (D) Analysis of DNA integrity in 7-d-lactating, 2- and 4-d-involuting wild-type and transgenic glands. On the left, 1-kb DNA ladder (Invitrogen) is shown.