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STAT5 and JAK2 phosphorylation levels in wild-type and MMTV-β1-cyto transgenic glands. (A) Protein extracts of 2- and 4-d-involuting mammary glands (100 μg of protein/lane) were analyzed by Western blotting for phospho-STAT5 and total STAT5 content. (B) Two milligrams of 2-d-involuting mammary gland protein extracts were immunoprecipitated with anti-JAK2 polyclonal antibody and analyzed by Western blotting for phospho-JAK2 and total JAK2 content.
