Figure 8.

Jasplakinolide stabilizes fibronectin matrix. Fibronectin-null cells were incubated overnight with 20 nM Texas Red (TR)-conjugated fibronectin. Cell were then either processed for immunofluorescence (Pulse, A) or were washed and then incubated with culture medium lacking fibronectin (Chase), in the absence (B and D) or presence (C and E) of 150 nM jasplakinolide, an actin stabilizing agent, for 24 h. Cells were fixed, permeabilized, and then incubated with FITC-phalloidin (to detect actin). TR-fibronectin (A–C) and actin (D and E) staining are shown. D and E are confocal images. Bar, 20 μm (A–C); bar, 10 μm (D and E).