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Tenascin-C inhibits RhoA localization to the cell membrane. NIH 3T3 fibroblasts were allowed to adhere to fibrin-FN+/−70Ten matrices for 1 h before lysis and ultracentrifugation to separate cytoplasmic (C) and membrane (M) fractions. Proteins were separated by SDS-PAGE, and immunoblots were probed with an anti-Rho mAb.
