Table 1.
Comparison of cell size and matrix contraction
| Fibrin-FN
|
Fibrin-FN+70Ten
|
|||
|---|---|---|---|---|
| Cell area (μm2) | Matrix contraction (%) | Cell area (μm2) | Matrix contraction (%) | |
| FAK+/+ | 812 ± 31 | 88 ± 2 | 314 ± 15 | 16 ± 0 |
| FAK+/++LPA | 346 ± 17 | 96 ± 3 | 174 ± 6 | 66 ± 0 |
| FAK −/− | 226 ± 12 | 36 ± 2 | 158 ± 10 | 16 ± 5 |
| FAK −/− +LPA | 126 ± 10 | 65 ± 5 | 162 ± 11 | 38 ± 3 |
Untreated fibroblasts derived from wild type (+/+) or FAK-deficient (−/−) mouse embryos or fibroblasts treated with LPA (+LPA) were mixed together with fibrin-FN or fibrin-FN+70Ten and allowed to polymerize in a 48-well dish. Matrix contraction data are expressed as the mean ± SEM of duplicate experiments. In parallel experiments, cells were plated on fibrin-FN+/− 70Ten matrices for 1 h, after which time areas for 50–75 cells from random fields were measured using IPlab Software and expressed in μm2 ± SD.