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. 2002 Oct;13(10):3627–3645. doi: 10.1091/mbc.E02-01-0061

Figure 7.

Figure 7

EB1-ΔC1-GFP displaces endogenous EB1 from microtubules. (A) Epitope mapping of the Transduction Laboratories anti-EB1 mAb. Purified GST, GST-EB1, and all EB1-deletion mutants were Western blotted and probed with the EB1 mAb. The antibody detected full-length EB1 and EB1 proteins with N-terminal deletions. Deletion of the last 50 aa of EB1 (EB1-ΔC1) destroyed the antibody epitope, which lies within the last 84 aa of EB1 (EB1-C84). (B–D) EB1-ΔC1-GFP competes with endogenous EB1 for binding to microtubules. COS-7 cells overexpressing EB1-ΔC1-GFP (asterisk) were processed for immunofluorescence with antibodies to GFP (B) and EB1 (C). (D) The merged image. Microtubule tip labeling for endogenous EB1 was absent in cells overexpressing EB1-ΔC1-GFP. Bar, 15 μm.