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Nucleotide exchange on ARF1. Myristoylated ARF1 was incubated at 37°C with [35S]GTPγS and either buffer only (▴), PC liposomes (●), or PC with 10% mixed phosphoinositides (□). At the indicated times, samples were quickly filtered through a nitrocellulose filter. After washing, the radioactivity on the filter, corresponding to GTPγS bound to ARF1, was counted.
