Figure 6.

Recruitment of pure AP-1 to peptidoliposomes. (A) Aliquots of the mixed adaptor preparation (lane 1) and of hydroxyapatite-purified AP-1 (lane 2) containing the same amount of AP-1 (as judged by immunoblot analysis) were separated by SDS-gel electrophoresis and stained with silver. AP-1 subunits β1, γ, and μ1 are indicated by filled arrowheads, whereas AP180 and AP-2 subunits α_a, α_c, β2, and μ2 are indicated by open arrowheads. (B) AP-1 recruitment assays were performed using liposomes made of PC with or without 1% PI(4,5)P₂ and exposing Lamp1Y (LY) or Lamp1A (LA) peptides in the presence of myristoylated ARF1 loaded with GMP-PNP or GDP. The analysis was performed as in Figure 3.