Figure 7.

Recruitment of AP-1 from cytosol. AP-1 recruitment assays were performed using brain cytosol (A) or adrenal gland cytosol (B), and peptidoliposomes made of soybean lipids (lanes 1–4), PC with 1% PI(4,5)P₂ (lanes 5–8), or pure PC (lanes 9–12), exposing Lamp1Y (LY) or Lamp1A (LA) peptides. Cytosol supplemented with purified ARF1 and GMP-PNP was incubated with the peptidoliposomes for 30 min at 37°C before separation by gradient centrifugation. (C) To determine the kinetics, AP-1 recruitment assays were performed using brain cytosol and liposomes exposing Lamp1Y peptides prepared of either PC alone (white bars) or PC containing 1% PI(4,5)P₂ (dark bars) at different incubation times (average and SD of 3 determinations).