Figure 3.

Detection of anti-CNG1 transcripts by RT-PCR and Northern blot. (A) RT-PCR detected the expression of anti-CNG1 in total RNAs prepared from brain tissue, glioma cell lines D247 and T98, and a commercial DNase-treated human brain total RNA (Invitrogen). Reverse transcription step was omitted in negative controls. (B) Multiple Tissue Northern blot analysis detected three anti-CNG1–related transcripts with molecular size of 5.5, 3.5, and 1.7 kb in many human brain regions. Each lane contained 2 μg poly(A) RNA. The probe for Northern blot was a strand-specific riboprobe derived from P2 region as labeled in Figure 2. The blot was reprobed with human β-actin gene to demonstrate that equal amounts of poly(A) RNA were loaded onto each lane.