Figure 8.

Cyclin D₁ gene promoter regulation by estradiol in HepG2 cells. Luciferase assay detection on HepG2 cells transfected with pXP2-D₁-2966-luciferase (-2966), pXP2-D₁Δ-944-luciferase (-944), pXP2-D₁Δ-848-luciferase (-848), and then treated 6 h with vehicle (C) or estradiol (E2) (10 nM) (a). Luciferase assay detection on cells transfected with pXP2-D₁Δ-254-luciferase (-254) and treated 6 h with vehicle (C) or E2 (10 nM) after 15 min of pretreatment with the MAP kinase pathway inhibitors PD98059 (PD) and U0126 (U0) (10 μM) (b). Data are expressed as percentage of variation with respect to the controls and are the means ± SD of four independent experiments. *P < 0.001, compared with respective control values (C), determined using Student's t test.