Figure 2.

Transcript ablation for nuclear encoded COX VI has no consequence for differentiation or the induction of kinetoplast-encoded COX I, II, or III. (A) Schematic representation of the RNAi construct pZJMCOXVI. The gene-coding region is integrated between opposing T7 promoters, generating sense and antisense transcripts. (B) Northern blot of COX VI, COX I, II, and III during the asynchronous differentiation of the monomorphic SMB line from bloodstream to procyclic forms. RNA samples were prepared from cells where COX VI RNAi was either not induced (− samples) or induced (+ samples). At the extreme left is shown RNA from control SMB cells untransfected with the pZJM COX VI construct (B) and the COX VI RNAi line grown in the absence of tetracycline (⁻; 0 h). At the extreme right is shown an established wild-type procyclic line grown in the presence of tetracycline (P).