Skip to main content
PMC home page
Biophysical Journal logoLink to Biophysical Journal
. 1998 Nov;75(5):2157–2162. doi: 10.1016/S0006-3495(98)77658-1

Adsorbed to a rigid substrate, dimyristoylphosphatidylcholine multibilayers attain full hydration in all mesophases.

J Katsaras 1
PMCID: PMC1299888  PMID: 9788909

Abstract

Whether hydrated from vapor or immersed in liquid water, aligned multibilayers of dimyristoylphosphatidylcholine adsorbed to a single mica "substrate" are shown by neutron diffraction to hydrate in all mesophases (e.g., Lbeta', Pbeta', and Lalpha) to the same extent as their liposomal counterparts suspended in liquid water. These data clearly demonstrate that the commonly accepted vapor pressure paradox does not exist.

Full Text

The Full Text of this article is available as a PDF (136.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Büldt G., Gally H. U., Seelig J., Zaccai G. Neutron diffraction studies on phosphatidylcholine model membranes. I. Head group conformation. J Mol Biol. 1979 Nov 15;134(4):673–691. doi: 10.1016/0022-2836(79)90479-0. [DOI] [PubMed] [Google Scholar]
  2. Franks N. P., Lieb W. R. The structure of lipid bilayers and the effects of general anaesthetics. An x-ray and neutron diffraction study. J Mol Biol. 1979 Oct 9;133(4):469–500. doi: 10.1016/0022-2836(79)90403-0. [DOI] [PubMed] [Google Scholar]
  3. Gibaud A, Cowley RA, McMorrow DF, Ward RC, Wells MR. High-resolution x-ray-scattering study of the structure of niobium thin films on sapphire. Phys Rev B Condens Matter. 1993 Nov 15;48(19):14463–14471. doi: 10.1103/physrevb.48.14463. [DOI] [PubMed] [Google Scholar]
  4. Henderson R. The structure of the purple membrane from Halobacterium hallobium: analysis of the X-ray diffraction pattern. J Mol Biol. 1975 Apr 5;93(2):123–138. doi: 10.1016/0022-2836(75)90123-0. [DOI] [PubMed] [Google Scholar]
  5. Janiak M. J., Small D. M., Shipley G. G. Nature of the Thermal pretransition of synthetic phospholipids: dimyristolyl- and dipalmitoyllecithin. Biochemistry. 1976 Oct 19;15(21):4575–4580. doi: 10.1021/bi00666a005. [DOI] [PubMed] [Google Scholar]
  6. Katsaras J. Highly aligned lipid membrane systems in the physiologically relevant "excess water" condition. Biophys J. 1997 Dec;73(6):2924–2929. doi: 10.1016/S0006-3495(97)78320-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Katsaras J., Jeffrey K. R., Yang D. S., Epand R. M. Direct evidence for the partial dehydration of phosphatidylethanolamine bilayers on approaching the hexagonal phase. Biochemistry. 1993 Oct 12;32(40):10700–10707. doi: 10.1021/bi00091a021. [DOI] [PubMed] [Google Scholar]
  8. Katsaras J., Raghunathan V. A., Dufourc E. J., Dufourcq J. Evidence for a two-dimensional molecular lattice in subgel phase DPPC bilayers. Biochemistry. 1995 Apr 11;34(14):4684–4688. doi: 10.1021/bi00014a023. [DOI] [PubMed] [Google Scholar]
  9. Katsaras J., Stinson R. H. High-resolution electron density profiles reveal influence of fatty acids on bilayer structure. Biophys J. 1990 Mar;57(3):649–655. doi: 10.1016/S0006-3495(90)82583-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Katsaras J., Yang D. S., Epand R. M. Fatty-acid chain tilt angles and directions in dipalmitoyl phosphatidylcholine bilayers. Biophys J. 1992 Oct;63(4):1170–1175. doi: 10.1016/S0006-3495(92)81680-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lis L. J., McAlister M., Fuller N., Rand R. P., Parsegian V. A. Interactions between neutral phospholipid bilayer membranes. Biophys J. 1982 Mar;37(3):657–665. [PMC free article] [PubMed] [Google Scholar]
  12. Podgornik R., Parsegian V. A. On a possible microscopic mechanism underlying the vapor pressure paradox. Biophys J. 1997 Feb;72(2 Pt 1):942–952. doi: 10.1016/s0006-3495(97)78728-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Raghunathan VA, Katsaras J. Structure of the Lc' phase in a hydrated lipid multilamellar system. Phys Rev Lett. 1995 May 29;74(22):4456–4459. doi: 10.1103/PhysRevLett.74.4456. [DOI] [PubMed] [Google Scholar]
  14. Robinson IK. Crystal truncation rods and surface roughness. Phys Rev B Condens Matter. 1986 Mar 15;33(6):3830–3836. doi: 10.1103/physrevb.33.3830. [DOI] [PubMed] [Google Scholar]
  15. Sinha SK, Sirota EB, Garoff S, Stanley HB. X-ray and neutron scattering from rough surfaces. Phys Rev B Condens Matter. 1988 Aug 1;38(4):2297–2311. doi: 10.1103/physrevb.38.2297. [DOI] [PubMed] [Google Scholar]
  16. Smith GS, Sirota EB, Safinya CR, Clark NA. Structure of the L beta phases in a hydrated phosphatidylcholine multimembrane. Phys Rev Lett. 1988 Feb 29;60(9):813–816. doi: 10.1103/PhysRevLett.60.813. [DOI] [PubMed] [Google Scholar]
  17. Stümpel J., Eibl H., Nicksch A. X-ray analysis and calorimetry on phosphatidylcholine model membranes. The influence of length and position of acyl chains upon structure and phase behaviour. Biochim Biophys Acta. 1983 Jan 19;727(2):246–254. doi: 10.1016/0005-2736(83)90410-8. [DOI] [PubMed] [Google Scholar]
  18. Torbet J., Wilkins M. H. X-ray diffraction studies of lecithin bilayers. J Theor Biol. 1976 Oct 21;62(2):447–458. doi: 10.1016/0022-5193(76)90129-6. [DOI] [PubMed] [Google Scholar]
  19. Tristram-Nagle S., Petrache H. I., Suter R. M., Nagle J. F. Effect of substrate roughness on D spacing supports theoretical resolution of vapor pressure paradox. Biophys J. 1998 Mar;74(3):1421–1427. doi: 10.1016/S0006-3495(98)77854-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tristram-Nagle S., Zhang R., Suter R. M., Worthington C. R., Sun W. J., Nagle J. F. Measurement of chain tilt angle in fully hydrated bilayers of gel phase lecithins. Biophys J. 1993 Apr;64(4):1097–1109. doi: 10.1016/S0006-3495(93)81475-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Zhang R, Sun W, Tristram-Nagle S, Headrick RL, Suter RM, Nagle aJ. Critical Fluctuations in Membranes. Phys Rev Lett. 1995 Apr 3;74(14):2832–2835. doi: 10.1103/PhysRevLett.74.2832. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES