Figure 2.

Intraclonal diversification in synovial germinal centre. Genealogical trees showing the diversification of a DPL23 (Vλ 3 germline gene) and an O18 (Vκ 1 germline gene) rearrangement. Numbered circles represent isolated sequences. The DPL23 rearrangement was isolated from two independent polymerase chain reactions (prefixes 1 and 2), and the O18 rearrangement was from serial sections 1, 2, 4 and 5. Numbers beside the lines refer to the number of nucleotide exchanges by which one sequence differs from the other sequences.