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Expression of PEST Mutants in COS-1 Cells. A) COS-1 cells were transiently transfected with cDNA encoding the various PEST mutants in both WT and ΔF508 background. Whole cell extracts were subjected to SDS-PAGE and immunoblot analysis (monoclonal antibody against the A52 tag) as described in 'Materials and Methods'. B) An over-exposure of the immunoblot showed the disappearance of the CFTR 100 kDa degradation product with the introduction of the poly-valine constructs.
