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. 2002 Sep 26;3:14. doi: 10.1186/1471-2199-3-14

Figure 4.

Figure 4

Effect of magnesium ions on binding of *VLF-1 to three-way junction DNA and cruciform DNA. Reaction mixtures (10 μl) containing 0.004 pmol 32P-labeled oligonucleotide probe and 35 ng of *VLF-1 were incubated for 20 min at 23°C, fixed with 0.5% glutaraldehyde, and analyzed by electrophoresis in a native 6% polyacrylamide gel as described in Materials and Methods. Reactions shown in lanes 1–6 contained three-way junction DNA (substrate IV), those shown in lanes 7–12 contained cruciform DNA (substrate V). No protein was added to the reactions shown in lanes 1 and 7. The final concentrations of MgCl2 (mM) were as indicated.