Figure 3:

Overall Reprogramming of the Immune Microenvironment in Cervical Cancer Patients upon Chemoradiotherapy. (A) UMAP visualization highlighting immune cell populations before (pre-RT) and after CRT (on-RT#2), only patients with both time points were included (CE322, CE323, CE326, CE331). (B) K-nearest neighbors (k-NN) differential abundance analysis comparing pre-RT and on-RT#2 samples from Cohort 2 and the results were overlapped using the UMAP (left) and visualized as bee swarm plot (right) to illustrates the main differences. (C) Differential gene expression analysis identifying CRT-induced changes across immune cell types. (D) Top 5 gene ontology terms enriched upon CRT across immune cell types, see Table S2. (E-K) Network plot of top significant upstream regulators (P<10⁻⁴) of immune cells pre-RT vs on-RT#2 time points, the inner nodes illustrate groups of regulators sharing genetic influences and the outer nodes represent contributing marker genes, color indicate the cell type; heatmaps for members of all nodes are presented to illustrate direction. (E) Neutrophils. (F) pDCs, DCs and monocytes. (G) Macrophages. (H) NK cells (I) CD4⁺ and CD8⁺ T-cells. (J) T_regs (K) Plasma and B cells.