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Recombinant proteins used in the tandem PRRS ELISA. (A) N-proteins of US PRRSV (124 amino acids) and EU-PRRSV (129 amino acids), C- and N-terminally fused to pQE31 vector-derived sequences, are shown. The hexahistidine tag used for nickel chelate affinity chromatography is underlined. (B) Polypeptide composed of 29 amino acid residues expressed from empty pQE31 vector plasmid and used as mock antigen in the tandem PRRS ELISA.
