FIG. 4.

Intracellular cytokine expression in splenic macrophages from athymic nude mice under different stimuli. Expression of IL-6 (A and D), TNF-α (B and E), and IL-12 (C and F) was determined. (A through C) Cytokine production was induced by tilorone treatment 24 h before the assay. (D through F) Mice were depleted of NK cells by intraperitoneal injection of anti-asialo-GM1 on the indicated days and then treated with tilorone 24 h before the assay. Percentages reflect cytokine-positive cells. Results are from one representative experiment out of three performed with cells from different mice.