TABLE 3.

Index values obtained from DBS before and after storage

Sample	Index values from HSV-1 assay on:			Index values from HSV-2 assay on:
	Original DBS	Frozen DBSa	FR/RT DBSb	Original DBS	Frozen DBS	FR/RT DBS
1	0.5	0.4	0.4	0.2	0.1	0.2
2	4.8c	5.5	4.4	0.3	0.0	0.2
3	4.8	5.4	5.0	3.2	1.3	1.5
4	0.4	0.2	0.2	0.4	0.2	0.2
5	5.3	5.3	4.5	1.5	0.9	0.6
6	1.1	1.0	0.6	0.4	0.1	0.1
7	5.2	5.3	5.4	0.5	0.1	0.1
8	0.2	0.2	0.2	0.5	0.0	0.0
9	1.1	0.8	0.6	0.4	0.1	0.1
10	1.0	1.1	1.1	0.3	0.1	0.2
11	1.5	1.2	1.0	11.8	8.6	8.7
12	0.2	0.1	0.2	0.4	0.1	0.1
13	0.2	0.1	0.2	0.2	0.0	0.1
14	4.4	4.8	4.4	0.6	0.1	0.2
15	4.6	6.7	5.1	0.3	0.1	0.1
16	4.8	6.2	3.6	0.6	0.1	0.1
17	4.8	3.6	3.1	0.1	0.1	0.1
18	5.1	5.6	4.0	0.5	0.2	0.2
19	4.8	4.5	3.8	0.4	0.1	0.1
20	5.2	6.3	6.2	0.3	0.1	0.1
21	5.0	6.3	6.0	0.3	0.0	0.1
22	4.3	3.3	2.4	9.4	6.6	5.4
Mean	3.15	3.36	2.84	1.48	0.86	0.84
% REACd		107	91		58	57

^aDBS stored for 1 year at −20°C.

^bDBS stored for 1 year at −20°C and then at room temperature for 30 days.

^cBoldface type indicates a positive index value.

^d% REAC, percent reactivity of the mean index values of stored samples compared to the mean index values of samples before storage for the respective type-specific antibody ELISA.