FIG. 2.

Effect of HIV-1 Nef protein, LPS, and IL-10 on mCD14 expression and the production of sCD14. PBMC (2 × 10⁶ cells/ml) or monocytes (1.0 × 10⁶ cells/ml) from healthy individuals (n = 6) were cultured with LPS (1 μg/ml), HIV-Nef protein (2 μg/ml), and IL-10 (1 ng/ml) for 48 h. (A) Cells were stained with FITC-labeled anti-CD14 antibodies and subjected to flow cytometric analysis. Culture supernatants were removed after 48 h of culture, and sCD14 was measured by ELISA. (B) Release of sCD14 from PBMC (2 × 10⁶ cells/ml) stimulated with LPS and IL-10. (C) Release of sCD14 from monocytes (1.0 × 10⁶ cells/ml) stimulated with LPS and HIV-Nef.