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[Preprint]. 2026 Mar 18:2026.02.21.707172. [Version 3] doi: 10.64898/2026.02.21.707172

Figure 4. Reln haploinsufficiency enhances acute fentanyl-induced locomotion and dorsal striatal Fos immunoreactivity without altering conditioned place preference.

Figure 4.

(A) Schematic of the CPP paradigm. (B) Change in percent time spent in the fentanyl-paired room at 20 μg/kg (left) or (80 μg/kg or (right). (C) Average locomotor speed (total distance / total time; meter / minute) during the first CPP conditioning session following acute fentanyl injection at 20 μg/kg (left) or 80 μg/kg or (right). (D) Representative fluorescent image of the dorsal striatum from WT (top) and Reln+/− (bottom) mice following acute injection (20 μg/kg). Fos immunoreactivity is shown in red; DAPI nuclear counterstain is in blue. Scale bar, 500um. (E) Quantification of Fos+ cells as a proportion of total DAPI+ cells in the dorsal striatum of a different cohort of mice following acute fentanyl injection (20 μg/kg, i.p.). Data are mean ± SEM. WT, n=4; Reln+/−, n=4 mice. *p<0.05.