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. 2005 Nov 30;33(21):6723–6732. doi: 10.1093/nar/gki961

Table 1.

Thermodynamic and kinetic parameters of (G3TTA)3G3 at 20°C in different experimental conditions

KA (M−1) <Γ> (s) kobs (M−1 s−1) Deq (nM) Qf (nM) Deq/Q0
Control 3.86 × 107 9340 10.7 × 10−5 12.4 17.6 0.41
5% EG 2.4 × 107 12284 8.14 × 10−5 9.8 20.2 0.33
10% EG 1.2 × 107 12318 8.11 × 10−5 6.6 23.4 0.22
5% GLY 2.6 × 107 12518 7.98 × 10−5 10.2 19.8 0.34
10% GLY 1.3 × 107 12563 7.95 × 10−5 6.9 23.1 0.23
1:5 (G:TMPyP4) 2.7 × 107 12987 7.7 × 10−5 10.4 19.6 0.35

KA is the equilibrium constant for telomeric duplex formation. <Γ> is the mean time constant. kobs is the observed rate constant. Deq, Qf and Deq/Q0 are duplex, free quadruplex concentrations and fraction of duplex at equilibrium when equimolar concentration of quadruplex and complementary strand (30 nM) are mixed together in 50 mM MES buffer, pH 7 with 100 mM KCl.