Figure 5.
Effect of various deletions in CtBP1 on its interaction with Glis2. The reporter plasmids (UAS)5-LUC and phRL-SV40 were co-transfected with pM-Glis2 and various pVP16-CtBP1 deletions mutants (A) in CHO cells, as indicated in the graph. After 48 h, cells were assayed for luciferase reporter activities as described in Materials and Methods. The relative reporter activity was calculated and plotted (B). The level of each CtBP1 fusion protein in total cellular extract was examined by western blot analysis with an anti-VP16 antibody (C). Data are means ± SEM of triplicate samples. FL, full-length CtBP1.