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. 2005 Dec 2;33(21):6805–6815. doi: 10.1093/nar/gki985

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© The Author 2005. Published by Oxford University Press. All rights reserved

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(A) CtBP1 suppressed Glis2(1–171)-mediated transcriptional activation. 293HT cells were co-transfected with (UAS)5-LUC, phRL-SV40, pM, pM-Glis2(1–171), and increasing amounts of pCMV-myc-CtBP1 (0–0.4 µg) as indicated. (B) CtBP1 inhibited Glis2(30–148) but not Glis1ΔN544 induced transactivation. 293HT cells were co-transfected with (UAS)5-LUC, phRL-SV40, pM, pM-Glis2(30–148) or pM-Glis1ΔN544, and pCMV-myc-CtBP1 (0.2 µg) as indicated. At 48 h after transfection, cells were assayed for LUC reporter activities. The relative LUC activity was calculated and plotted.