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. 2005 Dec 2;33(21):6805–6815. doi: 10.1093/nar/gki985

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Glis2 recruits CtBP1 and HDAC3. CHO cells were co-transfected with p3XFlag-CMV7.1, pEGFP-Glis2, p3XFlag-CMV-HDAC3 or pCMV-myc-CtBP1, as indicated. One part of the protein extracts was analyzed by western blot analysis (WB) for expression of EGFP-Glis2, Flag-HDAC-3 and myc-CtBP1. The other half was used for immuno-pulldown analysis as described in Materials and Methods. Proteins bound to anti-Flag M2 affinity resin were analyzed by western blot analysis using anti-GFP or anti-myc antibodies (Flag-IP/WB).