Table 1.
Glis2 expression enhances the nuclear localization of CtBP1
| Transfected-1 cells | EGFP-Glis2 | myc-CtBP1 | ||||
|---|---|---|---|---|---|---|
| %C | %N | %C + N | %C | %N | %C + N | |
| EGFP-Glis | 0 | 92.0 | 8.0 | — | — | — |
| myc-CtBP1 | — | — | — | 8.0 | 9.0 | 83.0 |
| EGFP-Glis2 + myc-CtBP1 | 0 | 94.0 | 6.0 | 2.0 | 71.0 | 27.0 |
Experiment was carried out as described in the legend of Figure 10A. Randomly, 100 cells were selected and the subcellular localization of CtBP1 or Glis2 determined. The percentage of cells containing Glis2 (EGFP fluorescence) or CtBP1 (Alexa Fluor® 594 fluorescence) predominantly in the nucleus (%N), cytoplasm (%C) or both (%N + C) was calculated. In the case of COS-1 cells co-transfected with both pEGFP-Glis2 and pCMV-myc-CtBP1, only cells were counted that contained both Glis2 and CtBP1.