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. 2005 Dec 2;33(21):6823–6836. doi: 10.1093/nar/gki987

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Evaluation of the performance of the Alu-based control reaction compared with a single-copy control reaction. Serial 1:25 dilutions of bisulfite-converted, M.SssI-treated DNA were used to compare the Alu and COL2A1 control reactions by real-time PCR. The fluorescence is plotted versus the PCR cycle number for both reactions and each sample dilution is indicated.