Table 1.

Description of repetitive element MethyLight reaction information

Reaction ID	GenBank number	Amplicon start	Amplicon end	Forward primer sequence 5′ to 3′	Reverse primer sequence 5′ to 3′	Probe sequence 5′ to 3′a
ALU-C4	Consensus Seq., Figure 1	1	98	GGTTAGGTATAGTGGTTTATATTTGTAATTTTAGTA	ATTAACTAAACTAATCTTAAACTCCTAACCTCA	CCTACCTTAACCTCCC-MGBb
ALU-M1c	Y07755	5059	5164	ATTATGTTAGTTAGGATGGTTTCGATTTT	CAATCGACCGAACGCGA	CCCAACACTTTAAAAAACCGAAATAAATAAATCACGA
ALU-M2	Consensus Seq., Figure 1	7	95	GCGCGGTGGTTTACGTTT	AACCGAACTAATCTCGAACTCCTAAC	AAATAATCCGCCCGCCTCGACCT
ALU-M3	Consensus Seq., Figure 1	133	210	ATTAGTCGGGCGTGGTGG	CCCGAATTCAAACGATTCTCC	CGCGTTTGTAATTTTAGTTATTCGGGAGGTTG
ALU-M5d	AC007256	156130	156221	GGTATGATGGCGTATGTTTGTAATTT	CGACTCACCACAACTTCCACC	AAACGATTCTCCTACCTCAACCTCCCGAA
ALU-U3	Consensus Seq., Figure 1	91	192	TGGTTAATATGGTGAAATTTTGTTTTTATT	TCCTACCTCAACCTCCCAAATAACT	CAAACACACACCACCACACCCAACTAATTT
LINE-1-M1	X52235	251	331	GGACGTATTTGGAAAATCGGG	AATCTCGCGATACGCCGTT	TCGAATATTGCGTTTTCGGATCGGTTT
LINE-1-U3	X52235	110	210	TTTATTAGGGAGTGTTAGATAGTGGGTG	CCTTACACTTCCCAAATAAAACAATACC	CACCCTACTTCAACTCATACACAATACACACACCC
SATα-M1	M38468	139	260	TGATGGAGTATTTTTAAAATATACGTTTTGTAGT	AATTCTAAAAATATTCCTCTTCAATTACGTAAA	TATCCCGTTTCCAACGAA-MGBb
SATα-U1	M38468	138	261	TTGATGGAGTATTTTTAAAATATATGTTTTGTAGT	AAATTCTAAAAATATTCCTCTTCAATTACATAAA	TTTATCCCATTTCCAACAAA-MGBb
SAT2-M1	X72623	1074	1153	TCGAATGGAATTAATATTTAACGGAAAA	CCATTCGAATCCATTCGATAATTCT	CGATTCCATTCGATAATTCCGTTT-MGBb

^aAll probes contain a 6FAM fluorophore and a BHQ-1 probe unless otherwise noted.

^bMGB refers to a Minor Groove Binder non-fluorescent quencher in the 3′ terminus of the probe (MGBNFQ).

^cThis MethyLight reaction was designed toward an Alu sequence in the S100A2 gene.

^dThis MethyLight reaction was designed toward an Alu sequence in the CASP8 gene.