Figure 7.

Effect of RAD51-3 or RAD51-5 mutation on the frequency of VSG switching. VSG switching frequencies for wild-type T.brucei cells (3174.2; WT) were compared with two independently generated heterozygous (+/− 1, 2) and homozygous mutants (−/− 1, 2) of either RAD51-3 or RAD51-5, in addition to rad51-3^−/− and rad51-5^−/− cell lines in which an intact copy of the mutated gene was re-expressed from the tubulin locus (−/−/+). Means of at least three independent experiments, plus SDs, are shown. P-values (determined by Student paired t-tests) of +/− cells' VSG switching frequencies relative to wt cells are indicated above the graph, as are the −/− cells' frequencies relative to the +/− cells from which they are derived, and the −/−/+ cells relative to the −/− mutants.