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. 2005 Dec 2;33(21):6906–6919. doi: 10.1093/nar/gki996

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Effect of RAD51-3 or RAD51-5 mutation on the VSG switching profile. (A) The form of VSG switching used in each cell line (see text) was determined by assaying for the expression of hygromycin and G418 resistance from the antibiotic marker genes (HYG and NEO, respectively) inserted around VSG-associated 70 bp repeat sequences (hatched box) in the active VSG221 ES (arrow denotes promoter) in strain 3174, as well as using PCR to assess whether HYG, NEO or VSG221 had been removed by gene conversion. (B) The percentage of clones that had undergone VSG switching by in situ switching, ES gene conversion/deletion or VSG gene conversion/deletion (see text) for each cell line are shown; the number of clones analysed (n) is indicated above the graph.