Table 2.
Percentages of RAD51-3 or RAD51-5 mutant cells displaying RAD51 foci following 18 h growth in phleomycin (BLE; concentrations are in µg ml−1), and the number of foci that are visible
| [BLE] | No. of foci | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | 4 | 5 | 6 | L | ||
| RAD51-3 | |||||||||
| Wild type | 1.5 | 35.0 | 28.2 | 21.4 | 10.3 | 3.4 | 3.4 | 0.9 | 0.9 |
| 1+/− | 1.5 | 27.4 | 30.8 | 18.8 | 15.4 | 6.0 | 1.7 | 0.0 | 0.0 |
| 1−/− | 0.3 | 81.7 | 7.3 | 5.5 | 5.5 | 0.0 | 0.0 | 0.0 | 0.0 |
| 1−/− | 1.5 | 63.8 | 18.1 | 12.1 | 3.4 | 2.6 | 0.0 | 0.0 | 0.0 |
| 2+/− | 1.5 | 22.2 | 26.5 | 18.8 | 14.5 | 4.3 | 1.7 | 0.9 | 1.7 |
| 2−/− | 0.3 | 59.0 | 19.7 | 12.0 | 4.3 | 0.9 | 0.9 | 0.0 | 0.0 |
| 2−/− | 1.5 | 52.1 | 24.8 | 8.5 | 0.9 | 1.7 | 0.9 | 0.0 | 0.9 |
| RAD51-5 | |||||||||
| Wild type | 1.5 | 22.3 | 16.1 | 24.1 | 17.9 | 11.6 | 4.5 | 1.8 | 1.8 |
| 1+/− | 1.5 | 19.8 | 24.3 | 33.3 | 14.4 | 5.4 | 1.8 | 0.9 | 0.0 |
| 1−/− | 0.5 | 67.3 | 18.3 | 16.3 | 5.8 | 1.0 | 0.0 | 0.0 | 0.0 |
| 1−/− | 1.5 | 59.5 | 18.0 | 9.0 | 2.7 | 0.9 | 0.9 | 0.0 | 0.9 |
| 2+/− | 1.5 | 27.9 | 32.4 | 19.8 | 13.5 | 2.7 | 4.5 | 3.6 | 3.6 |
| 2−/− | 0.5 | 64.0 | 22.5 | 7.2 | 3.6 | 0.9 | 0.0 | 0.9 | 0.9 |
| 2−/− | 1.5 | 64.9 | 23.4 | 3.6 | 1.8 | 2.7 | 0.0 | 0.0 | 0.0 |
L represents >6 foci, +/− denotes heterozygous mutants, and −/− denotes homozygous mutants).