Table 2.
Summary of real-time PCR data
| Sample | Chromosome | Position (Mb) | Method | Copy number (real time PCR) | Copy number (MIP) |
|---|---|---|---|---|---|
| NA19193 | 12 | 31.3 | Taqman | 2.8 ± 0.69a | 2.4 |
| NA18573 | 15 | 71.6 | Taqman | 1.86 ± 0.31b | 2 |
| HCC1395 | 2 | 114.3 | SYBR | 0.09 ± 0.006c | 0.06 |
| HCC1395 | 6 | 105.6 | SYBR | 0.12 ± 0.008 | 0.06 |
| HCC1395 | 5 | 107.3 | Taqman | 0.05 ± 0.016 | 0.05 |
| HCC1395 | 13 | 59.2 | Taqman | 0.05 ± 0.012 | 0.02 |
| UACC-812 | 17 | 35.1 | SYBR | 42.3 ± 1.4c | 15.1 |
| UACC-812 | 17 | 12.5 | SYBR | 7.7 ± 0.16 | 4.4 |
| UACC-812 | 17 | 44.2 | SYBR | 8.4 ± 0.032 | 4.7 |
| UACC-812 | 17 | 3.4 | SYBR | 9.4 ± 1.4 | 3.4 |
| UACC-812 | 17 | 24.4 | SYBR | 8.0 ± 0.44c | 5.6 |
All the real-time PCR was done in duplicates except when indicated.
aEstimated from 40 replicates. Therefore the estimated standard error of the average is 0.11.
bEstimated from 6 replicates. Therefore the estimated standard error of the average is 0.13.
cDetermined by average of two duplicates from two different real-time PCR loci totaling four reactions.