Figure 3. Roles of the DNA damage response (DDR) pathway and p53 in Ras^G12V-induced nurse cell death.

(A) Representative ovaries (DAPI staining). Scale bars: 200 μm. (B) Quantification data. 30 ovaries from 3-day-old flies were quantified for each genotype. Statistical significance was determined using one-way ANOVA: **** (p<0.0001). (C and E) Representative samples. Scale bars: 20 μm. (D) Schematic cartoon for uev1a-flag knock-in.

Figure 3—figure supplement 1. Oncogenic Ras^G12V intrinsically triggers nurse cell death.

(A) Representative ovaries (DAPI staining). Scale bars: 200 μm. (B) Quantification data. 30 ovaries from 3-day-old flies were quantified for each genotype. Statistical significance was determined using the t test: **** (p<0.0001).

Figure 3—figure supplement 2. Uev1A is expressed in stretch follicle cells.

Representative ovaries.

Figure 3—figure supplement 3. Uev1A does not directly degrade the Ras^G12V oncoproteins.

These experiments were performed at 29°C. All images are of the same magnification.