Figure 2.

Effects of temperature on V_rest, EJPs, and voltage-clamp assessment of temperature-sensitive current in gm5b

(A) Current-clamp recording of the muscle fiber from 11°C to 25°C while the lgn is stimulated at 5 Hz for 2 s (gray dashed line is V_rest).

(B) The resting membrane voltage hyperpolarizes monotonically with warming.

(C) Example current traces acquired while stepping the membrane potential (from −130 mV to −60 mV over 2 s, left) at 11°C (center) and 21°C (right).

(D) Temperature-dependence of the current from 5°C to 21°C (from black to red).

(E and F) Currents at −120 mV and −65 mV show the inward and outward currents in warm solutions. The horizontal and vertical lines represent the median and the standard deviation in (B, E, and F).