Fig. 3. Evaluation of colourimetric dyes. (A) Pictures of cLAMP reactions using pH sensitive dyes, showing negative (NEG) and positive (POS) amplification after incubation at 63 °C. Real-time data showing TTP values against dye concentration, including as reference (ref) a reaction without dye. (B) Pictures of cLAMP reactions using metal indicators, showing negative (NEG) and positive (POS) amplification after incubation at 63 °C. Real-time data showing TTP values against indicator concentration, including as reference (ref) a reaction without dye. (C) Cross-titration of pH sensitive dyes with metal indicators eriochrome black T (EBT), calmagite (C), and xylidyl blue (XB). Pictures of cLAMP reactions, showing negative (NEG) and positive (POS) amplification after incubation at 63 °C. Real-time data showing TTP values against dye concentration, including as reference (ref) a reaction without dye. (D) MgSO4 titration (0–14 mM) in cLAMP comparing its effect in the presence of pH and metal indicators and the combination of both.
