Fig. 4. Analytical sensitivity of colourimetric lyophilised reactions. Pictures and standard curves (TTP against RNA concentration) of lyo-cLAMP reactions with the following indicators: (A) phenol red 100 µM, (B) phenol red 100 µM with eriochorme black T 75 µM, (C) thymol blue 150 µM with eriochorme black T 100 µM, (D) bromothymol blue 100 µM with eriochorme black T 75 µM, and (E) bromothymol blue 100 µM with calmagite 50 µM. Pictures showing negative (NEG) and positive (POS) amplification after incubation at 63 °C, including liquid cLAMP as reference. (F) ΔpH data obtained from lyo-cLAMP (MM1 to MM5) positive amplification reactions before and after amplification. Vertical line denotes the average, and the standard deviation is shadowed.
