Fig. 5. Evaluation of lyo-cLAMP with residual respiratory specimens from nasal, nasopharyngeal or buccal swabs. Pictures of lyo-cLAMP reactions after 35 min incubation at 63 °C using: (A) lyo-cLAMP MM1 (phenol red 100 µM), (B) lyo-cLAMP MM2 (phenol red 100 µM with eriochorme black T 75 µM), (C) lyo-cLAMP MM3 (thymol blue 150 µM with eriochorme black T 100 µM), (D) lyo-cLAMP MM4 (bromothymol blue 100 µM with eriochorme black T 75 µM), and (E) lyo-cLAMP MM5 (bromothymol blue 100 µM with calmagite 50 µM). (F) PCR Cq values distribution using CDC assays targeting SARS-CoV-2 (N1). Extracted nucleic acids from nasal, nasopharyngeal or buccal swabs (N = 15).
