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H463G mutation does not affect acidification-induced activation shift in the Kv1.5 R487Y channel. (A) Activation at pH 7.3 and 6.6 in Kv1.5 R487Y. V1/2 values: −19.1 ± 1.0 (pH 7.3, n = 4) and −9.5 ± 1.5 (pH 6.6, n = 5). (B) Activation at pH 7.3 and 6.6 in Kv1.5 H463G R487Y. V1/2 values: −18.7 ± 0.7 (pH 7.3, n = 3) and −9.0 ± 1.5 (pH 6.6, n = 3). Currents were recorded in 0 external K+.
