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. 2003 Jan;84(1):509–522. doi: 10.1016/S0006-3495(03)74870-X

FIGURE 5.

FIGURE 5

Dynamic surface tensions of the mixture of IgG and Protein A solutions. In the mixture, the concentration of IgG is fixed at 1.67 × 10−6 M, and the concentration of Protein A is 5.0 × 10−3 , 0.05, 0.10, 0.50, 0.835, 1.0, and 2.0 μM, which correspond to molar ratios of Protein A to IgG at 1:334, 1:33.4, 1:16.7, 1:3.34, 1:2, 1:1.67, and 1:0.835, respectively. The dynamic surface tension measurements started immediately after mixing, and experimental duration was 5 h. It is noted that the highest surface tension is observed at the molar ratio of Protein A to IgG of ∼1:2.