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. 2003 Jan;84(1):665–670. doi: 10.1016/S0006-3495(03)74886-3

FIGURE 3.

FIGURE 3

NPC diameter increases after incubation with molecules that bind with low affinity to FG-repeats. Plot of NPC diameter under the given conditions. NPC diameter increases after low affinity binding to FG-repeats by I178D (1 μM; 2 min), 1,2-cyclohexanediol (2.0%; 2–10 min), 1,4-cyclohexanediol (2.0%; 2–10 min) and 1,6-hexanediol (2.0%; 2–10 min). The solid triangles represent the extent of reversion in NPC diameter after washing out of 1,2- and 1,4-cyclohexandiol for 10 min. Washing involves prolonged handling of the nuclei as is evidenced by the increase in the size of the error bars representing the greater variability in NPC dimensions. The sample sizes are identical to those in Fig. 2 with the additions of 1,2-cyclohexanediol wash (85/2/2) and 1,4-cyclohexanediol wash (162/3/2).