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. 2003 Feb;84(2):977–986. doi: 10.1016/S0006-3495(03)74914-5

FIGURE 1.

FIGURE 1

Macroscopic fast vacuolar (FV) currents in vacuoles of Beta vulgaris at different cytosolic and vacuolar K+ concentrations. Original recordings and correspondent current-voltage relations on large cytosolic (a, C ∼ 1 pF) and vacuolar (b, C ∼ 0.5 pF) side-out patches. From holding value of −40 mV, the voltage was stepped to ± 160 mV with 20-mV increment; at the end of each step the voltage was switched to −100 mV. Pipette solution contained 100 mM K+; cytosolic (a) and vacuolar (b) K+ concentration (in mM) was varied by bath perfusion as indicated. In the case of solution containing 90 mM NMDG+, voltage was corrected for the liquid junction potential between bath and reference electrode.